Sonderkolloquium, Frau Dr. Azra Bahadori, Niels-Bohr-Insitut, Universität Kopenhagen / am 27.03.2017

27.03.2017 von 16:45 bis 17:30

Institut für Materialwissenschaft, "Aquarium", Geb. D., Kaiserstraße 2, 24143 Kiel

Titel: Optically controlled and selective fusion of eukaryotic cells and/or synthetic vesicles

Abstract:

Membrane fusion is a ubiquitous phenomenon in biology facilitating a range of fundamental processes including intracellular communications, intercellular transport and fertilization. However, there exist several high energy barriers which have to be lowered in order to induce membrane fusion. I will discuss our new and highly selective strategy for triggering membrane fusion by means of optical trapping of plasmonic nanoparticles. I will present experimental data of lipid mixing and cargo mixing which indicates complete fusion. Using a relevant quantitative model which describes the translational diffusion in the membrane of spherical shells, we also found that our experimentally obtained time scales for membrane mixing are consistent with the time scales extracted from the model. I will also show data which proves that our membrane fusion method is biologically non-invasive; therefore can be of particular importance in targeted drug delivery and nano-medicine. Finally, due to the crucial role of optical tweezers in our method of triggering membrane fusion, I will introduce our steerable dual-beam optical tweezers setup as a powerful facility which has been used to conduct
advanced nanomanipulations in our group.

Prof. Christine Selhuber-Unkel

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